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Servicebio Inc tbst g0004
Tbst G0004, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tbst+g0004/tbst+g0004/pm40660286-157-8-10
Average 90 stars, based on 1 article reviews
tbst g0004 - by Bioz Stars, 2026-10
90/100 stars

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Incubation:

Article Title: Glycolysis-Derived Lactate Induces ACSL4 Expression and Lactylation to Activate Ferroptosis during Intervertebral Disc Degeneration.
Article Snippet: Next, the slides or NPCs were washed with TBST (G0004; Servicebio), followed by incubation with the secondary antibodies, FITC/Cy3 IgG (GB21401 and GB22301; Servicebio).

Article Title: Esketamine Prevents Postoperative Emotional and Cognitive Dysfunction by Suppressing Microglial M1 Polarization and Regulating the BDNF-TrkB Pathway in Ageing Rats with Preoperative Sleep Disturbance.
Article Snippet: After washing with TBST (catalogue: G0004, Servicebio, Wuhan, China), we incubated the blots with anti-mouse horseradish peroxidase (HRP)-conjugated IgG antibody H&L (1:1000, catalogue: BA1050, Boster, Wuhan, China), anti-rabbit HRP-conjugated IgG antibody H&L (1:1000, catalogue: BA1054, Boster, Wuhan, China), or anti-goat HRP-conjugated IgG antibody H&L (1:1000, catalogue: BA1060, Boster, Wuhan, China) for 1 h at room temperature.

Article Title: Glycolysis‐Derived Lactate Induces ACSL4 Expression and Lactylation to Activate Ferroptosis during Intervertebral Disc Degeneration
Article Snippet: Next, the slides were washed with TBST (G0004; Servicebio), followed by the incubation with secondary antibodies, HRP‐conjugated goat anti‐rabbit IgG (GB23303; ServiceBio).

Article Title: Glycolysis-Derived Lactate Induces ACSL4 Expression and Lactylation to Activate Ferroptosis during Intervertebral Disc Degeneration.
Article Snippet: Next, the slides were washed with TBST (G0004; Servicebio), followed by the incubation with secondary antibodies, HRPconjugated goat anti-rabbit IgG (GB23303; ServiceBio).

Article Title: Targeted Inhibition of cGAS/STING signaling induced by aberrant R-Loops in the nucleus pulposus to alleviate cellular senescence and intervertebral disc degeneration.
Article Snippet: For the IF staining of rat NPCs, after treatment, the cells were immobilized with 4% paraformaldehyde for 10 min, followed by permeabilization using 0.15% Triton X-100 for an additional 5 min, and subsequently washed three times with PBS, and then incubated with γH2AX (GB111841,Servicebio), p16INK4a(AF5484, Affinity; R23896, Zenbio), RnaseH1 (DF12084, Affinity), MMP3 (340612, ZenBio), ACAN (DF7561, Affinity), S9.6 (Kerafast, Cat # ENH001), Nucleolin (GB112016, Servicebio), ERCC5 (DF3143, Affinity), GLB1(DF6667, Adffinity), IL-1β (516288, Zenbio), TOMM20 (GB111481, Servicebio), STING (R25931, Zenbio; DF12090, Affinity), dsDNA (MA1-35346, Santa Cruz Biotechnology, Inc.), IL-6 (GB11117, Servicebio), Ki67(GB121141, Servicebio), and ERCC6 (DF15440, Affinity).

Article Title: Ganodermanontriol Suppresses the Progression of Lung Adenocarcinoma by Activating CES2 to Enhance the Metabolism of Mycophenolate Mofetil
Article Snippet: Subsequently, the sections were treated with 5% non-fat milk (abs9175, Absin, China) in 1×TBS with Tween-20 (TBST; G0004, Servicebio, China) at RT for 30 min. Then, the sections were incubated with CES2 antibody (PA5-102415, Thermo Fisher) at 4°C overnight, followed by hybridization with horseradish peroxidase (HRP)-conjugated mouse anti-rabbit IgG (D110065, Sangon Biotech, China) at RT for 1 h. After treatment with DAB (D12384, Sigma-Aldrich) and counterstaining with hematoxylin (G1004, Servicebio, China) in sequence, AXio Lab.A1 microscope (×100 magnification, Zeiss, ermany) was used to observe positive signals of CES2 in the sections.

Article Title: cGAS-STING pathway aggravates early cerebral ischemia-reperfusion injury in mice by activating NCOA4-mediated ferritinophagy.
Article Snippet: Stroke patients are often complicated by cerebral ischemia-reperfusion injury (CIRI) after the restoration of cerebral perfusion, and how to prevent CIRI at an early stage has received close attention.. The imbalance of iron metabolism is one of the essential factors in the aggravation of CIRI, and NCOA4-mediated ferritinophagy, as a critical pathway to regulate iron metabolism, is expected to be an effective intervention target.. We established a mouse model of cerebral ischemia-reperfusion (CIR) with NCOA4 silencing.

Hybridization:

Article Title: Glycolysis-Derived Lactate Induces ACSL4 Expression and Lactylation to Activate Ferroptosis during Intervertebral Disc Degeneration.
Article Snippet: Next, the slides or NPCs were washed with TBST (G0004; Servicebio), followed by incubation with the secondary antibodies, FITC/Cy3 IgG (GB21401 and GB22301; Servicebio).

Article Title: Esketamine Prevents Postoperative Emotional and Cognitive Dysfunction by Suppressing Microglial M1 Polarization and Regulating the BDNF-TrkB Pathway in Ageing Rats with Preoperative Sleep Disturbance.
Article Snippet: After washing with TBST (catalogue: G0004, Servicebio, Wuhan, China), we incubated the blots with anti-mouse horseradish peroxidase (HRP)-conjugated IgG antibody H&L (1:1000, catalogue: BA1050, Boster, Wuhan, China), anti-rabbit HRP-conjugated IgG antibody H&L (1:1000, catalogue: BA1054, Boster, Wuhan, China), or anti-goat HRP-conjugated IgG antibody H&L (1:1000, catalogue: BA1060, Boster, Wuhan, China) for 1 h at room temperature.

Article Title: Glycolysis‐Derived Lactate Induces ACSL4 Expression and Lactylation to Activate Ferroptosis during Intervertebral Disc Degeneration
Article Snippet: Next, the slides were washed with TBST (G0004; Servicebio), followed by the incubation with secondary antibodies, HRP‐conjugated goat anti‐rabbit IgG (GB23303; ServiceBio).

Article Title: Glycolysis-Derived Lactate Induces ACSL4 Expression and Lactylation to Activate Ferroptosis during Intervertebral Disc Degeneration.
Article Snippet: Next, the slides were washed with TBST (G0004; Servicebio), followed by the incubation with secondary antibodies, HRPconjugated goat anti-rabbit IgG (GB23303; ServiceBio).

Article Title: Targeted Inhibition of cGAS/STING signaling induced by aberrant R-Loops in the nucleus pulposus to alleviate cellular senescence and intervertebral disc degeneration.
Article Snippet: For the IF staining of rat NPCs, after treatment, the cells were immobilized with 4% paraformaldehyde for 10 min, followed by permeabilization using 0.15% Triton X-100 for an additional 5 min, and subsequently washed three times with PBS, and then incubated with γH2AX (GB111841,Servicebio), p16INK4a(AF5484, Affinity; R23896, Zenbio), RnaseH1 (DF12084, Affinity), MMP3 (340612, ZenBio), ACAN (DF7561, Affinity), S9.6 (Kerafast, Cat # ENH001), Nucleolin (GB112016, Servicebio), ERCC5 (DF3143, Affinity), GLB1(DF6667, Adffinity), IL-1β (516288, Zenbio), TOMM20 (GB111481, Servicebio), STING (R25931, Zenbio; DF12090, Affinity), dsDNA (MA1-35346, Santa Cruz Biotechnology, Inc.), IL-6 (GB11117, Servicebio), Ki67(GB121141, Servicebio), and ERCC6 (DF15440, Affinity).

Article Title: Ganodermanontriol Suppresses the Progression of Lung Adenocarcinoma by Activating CES2 to Enhance the Metabolism of Mycophenolate Mofetil
Article Snippet: Subsequently, the sections were treated with 5% non-fat milk (abs9175, Absin, China) in 1×TBS with Tween-20 (TBST; G0004, Servicebio, China) at RT for 30 min. Then, the sections were incubated with CES2 antibody (PA5-102415, Thermo Fisher) at 4°C overnight, followed by hybridization with horseradish peroxidase (HRP)-conjugated mouse anti-rabbit IgG (D110065, Sangon Biotech, China) at RT for 1 h. After treatment with DAB (D12384, Sigma-Aldrich) and counterstaining with hematoxylin (G1004, Servicebio, China) in sequence, AXio Lab.A1 microscope (×100 magnification, Zeiss, ermany) was used to observe positive signals of CES2 in the sections.

Article Title: cGAS-STING pathway aggravates early cerebral ischemia-reperfusion injury in mice by activating NCOA4-mediated ferritinophagy.
Article Snippet: Stroke patients are often complicated by cerebral ischemia-reperfusion injury (CIRI) after the restoration of cerebral perfusion, and how to prevent CIRI at an early stage has received close attention.. The imbalance of iron metabolism is one of the essential factors in the aggravation of CIRI, and NCOA4-mediated ferritinophagy, as a critical pathway to regulate iron metabolism, is expected to be an effective intervention target.. We established a mouse model of cerebral ischemia-reperfusion (CIR) with NCOA4 silencing.

Sequencing:

Article Title: Glycolysis-Derived Lactate Induces ACSL4 Expression and Lactylation to Activate Ferroptosis during Intervertebral Disc Degeneration.
Article Snippet: Next, the slides or NPCs were washed with TBST (G0004; Servicebio), followed by incubation with the secondary antibodies, FITC/Cy3 IgG (GB21401 and GB22301; Servicebio).

Article Title: Esketamine Prevents Postoperative Emotional and Cognitive Dysfunction by Suppressing Microglial M1 Polarization and Regulating the BDNF-TrkB Pathway in Ageing Rats with Preoperative Sleep Disturbance.
Article Snippet: After washing with TBST (catalogue: G0004, Servicebio, Wuhan, China), we incubated the blots with anti-mouse horseradish peroxidase (HRP)-conjugated IgG antibody H&L (1:1000, catalogue: BA1050, Boster, Wuhan, China), anti-rabbit HRP-conjugated IgG antibody H&L (1:1000, catalogue: BA1054, Boster, Wuhan, China), or anti-goat HRP-conjugated IgG antibody H&L (1:1000, catalogue: BA1060, Boster, Wuhan, China) for 1 h at room temperature.

Article Title: Glycolysis‐Derived Lactate Induces ACSL4 Expression and Lactylation to Activate Ferroptosis during Intervertebral Disc Degeneration
Article Snippet: Next, the slides were washed with TBST (G0004; Servicebio), followed by the incubation with secondary antibodies, HRP‐conjugated goat anti‐rabbit IgG (GB23303; ServiceBio).

Article Title: Glycolysis-Derived Lactate Induces ACSL4 Expression and Lactylation to Activate Ferroptosis during Intervertebral Disc Degeneration.
Article Snippet: Next, the slides were washed with TBST (G0004; Servicebio), followed by the incubation with secondary antibodies, HRPconjugated goat anti-rabbit IgG (GB23303; ServiceBio).

Article Title: Targeted Inhibition of cGAS/STING signaling induced by aberrant R-Loops in the nucleus pulposus to alleviate cellular senescence and intervertebral disc degeneration.
Article Snippet: For the IF staining of rat NPCs, after treatment, the cells were immobilized with 4% paraformaldehyde for 10 min, followed by permeabilization using 0.15% Triton X-100 for an additional 5 min, and subsequently washed three times with PBS, and then incubated with γH2AX (GB111841,Servicebio), p16INK4a(AF5484, Affinity; R23896, Zenbio), RnaseH1 (DF12084, Affinity), MMP3 (340612, ZenBio), ACAN (DF7561, Affinity), S9.6 (Kerafast, Cat # ENH001), Nucleolin (GB112016, Servicebio), ERCC5 (DF3143, Affinity), GLB1(DF6667, Adffinity), IL-1β (516288, Zenbio), TOMM20 (GB111481, Servicebio), STING (R25931, Zenbio; DF12090, Affinity), dsDNA (MA1-35346, Santa Cruz Biotechnology, Inc.), IL-6 (GB11117, Servicebio), Ki67(GB121141, Servicebio), and ERCC6 (DF15440, Affinity).

Article Title: Ganodermanontriol Suppresses the Progression of Lung Adenocarcinoma by Activating CES2 to Enhance the Metabolism of Mycophenolate Mofetil
Article Snippet: Subsequently, the sections were treated with 5% non-fat milk (abs9175, Absin, China) in 1×TBS with Tween-20 (TBST; G0004, Servicebio, China) at RT for 30 min. Then, the sections were incubated with CES2 antibody (PA5-102415, Thermo Fisher) at 4°C overnight, followed by hybridization with horseradish peroxidase (HRP)-conjugated mouse anti-rabbit IgG (D110065, Sangon Biotech, China) at RT for 1 h. After treatment with DAB (D12384, Sigma-Aldrich) and counterstaining with hematoxylin (G1004, Servicebio, China) in sequence, AXio Lab.A1 microscope (×100 magnification, Zeiss, ermany) was used to observe positive signals of CES2 in the sections.

Article Title: cGAS-STING pathway aggravates early cerebral ischemia-reperfusion injury in mice by activating NCOA4-mediated ferritinophagy.
Article Snippet: Stroke patients are often complicated by cerebral ischemia-reperfusion injury (CIRI) after the restoration of cerebral perfusion, and how to prevent CIRI at an early stage has received close attention.. The imbalance of iron metabolism is one of the essential factors in the aggravation of CIRI, and NCOA4-mediated ferritinophagy, as a critical pathway to regulate iron metabolism, is expected to be an effective intervention target.. We established a mouse model of cerebral ischemia-reperfusion (CIR) with NCOA4 silencing.

Microscopy:

Article Title: Glycolysis-Derived Lactate Induces ACSL4 Expression and Lactylation to Activate Ferroptosis during Intervertebral Disc Degeneration.
Article Snippet: Next, the slides or NPCs were washed with TBST (G0004; Servicebio), followed by incubation with the secondary antibodies, FITC/Cy3 IgG (GB21401 and GB22301; Servicebio).

Article Title: Esketamine Prevents Postoperative Emotional and Cognitive Dysfunction by Suppressing Microglial M1 Polarization and Regulating the BDNF-TrkB Pathway in Ageing Rats with Preoperative Sleep Disturbance.
Article Snippet: After washing with TBST (catalogue: G0004, Servicebio, Wuhan, China), we incubated the blots with anti-mouse horseradish peroxidase (HRP)-conjugated IgG antibody H&L (1:1000, catalogue: BA1050, Boster, Wuhan, China), anti-rabbit HRP-conjugated IgG antibody H&L (1:1000, catalogue: BA1054, Boster, Wuhan, China), or anti-goat HRP-conjugated IgG antibody H&L (1:1000, catalogue: BA1060, Boster, Wuhan, China) for 1 h at room temperature.

Article Title: Glycolysis‐Derived Lactate Induces ACSL4 Expression and Lactylation to Activate Ferroptosis during Intervertebral Disc Degeneration
Article Snippet: Next, the slides were washed with TBST (G0004; Servicebio), followed by the incubation with secondary antibodies, HRP‐conjugated goat anti‐rabbit IgG (GB23303; ServiceBio).

Article Title: Glycolysis-Derived Lactate Induces ACSL4 Expression and Lactylation to Activate Ferroptosis during Intervertebral Disc Degeneration.
Article Snippet: Next, the slides were washed with TBST (G0004; Servicebio), followed by the incubation with secondary antibodies, HRPconjugated goat anti-rabbit IgG (GB23303; ServiceBio).

Article Title: Targeted Inhibition of cGAS/STING signaling induced by aberrant R-Loops in the nucleus pulposus to alleviate cellular senescence and intervertebral disc degeneration.
Article Snippet: For the IF staining of rat NPCs, after treatment, the cells were immobilized with 4% paraformaldehyde for 10 min, followed by permeabilization using 0.15% Triton X-100 for an additional 5 min, and subsequently washed three times with PBS, and then incubated with γH2AX (GB111841,Servicebio), p16INK4a(AF5484, Affinity; R23896, Zenbio), RnaseH1 (DF12084, Affinity), MMP3 (340612, ZenBio), ACAN (DF7561, Affinity), S9.6 (Kerafast, Cat # ENH001), Nucleolin (GB112016, Servicebio), ERCC5 (DF3143, Affinity), GLB1(DF6667, Adffinity), IL-1β (516288, Zenbio), TOMM20 (GB111481, Servicebio), STING (R25931, Zenbio; DF12090, Affinity), dsDNA (MA1-35346, Santa Cruz Biotechnology, Inc.), IL-6 (GB11117, Servicebio), Ki67(GB121141, Servicebio), and ERCC6 (DF15440, Affinity).

Article Title: Ganodermanontriol Suppresses the Progression of Lung Adenocarcinoma by Activating CES2 to Enhance the Metabolism of Mycophenolate Mofetil
Article Snippet: Subsequently, the sections were treated with 5% non-fat milk (abs9175, Absin, China) in 1×TBS with Tween-20 (TBST; G0004, Servicebio, China) at RT for 30 min. Then, the sections were incubated with CES2 antibody (PA5-102415, Thermo Fisher) at 4°C overnight, followed by hybridization with horseradish peroxidase (HRP)-conjugated mouse anti-rabbit IgG (D110065, Sangon Biotech, China) at RT for 1 h. After treatment with DAB (D12384, Sigma-Aldrich) and counterstaining with hematoxylin (G1004, Servicebio, China) in sequence, AXio Lab.A1 microscope (×100 magnification, Zeiss, ermany) was used to observe positive signals of CES2 in the sections.

Article Title: cGAS-STING pathway aggravates early cerebral ischemia-reperfusion injury in mice by activating NCOA4-mediated ferritinophagy.
Article Snippet: Stroke patients are often complicated by cerebral ischemia-reperfusion injury (CIRI) after the restoration of cerebral perfusion, and how to prevent CIRI at an early stage has received close attention.. The imbalance of iron metabolism is one of the essential factors in the aggravation of CIRI, and NCOA4-mediated ferritinophagy, as a critical pathway to regulate iron metabolism, is expected to be an effective intervention target.. We established a mouse model of cerebral ischemia-reperfusion (CIR) with NCOA4 silencing.



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